Moreover, these authors narrowed down the Nup214 binding site to a 137-amino-acid segment in the N-terminal domain of Nup214 by performing several experiments, including rescue experiments with the digitonin-permeabilized Nup214-depleted cells in the presence of several fragments of recombinant Nup214, adenovirus infection of cells overexpressing different fragments of Nup154, and in vitro binding assays with purified hexon and recombinant Nup214 fragments. This evidence concerns the gene NUP214 and adenoviridae infectious disease.