We were not able to perform gene copy analysis of EGFR because of the limited sample volume, but EGFR gene amplification is a potential mechanism for EGFR activation, because amplification and activation of EGFR has been reported in tumors from patients with glioblastoma multiform (GBM), NSCLC, and breast cancer [21, 22]. This evidence concerns the gene EGFR and glioblastoma.