TP53 and neoplasm: The increased levels of nuclear p53 in tumor cells were described more than 3 decades ago but the relation with TP53 mutation became clear 10 years later and immunohistochemistry analysis of tumors using various p53 monoclonal antibodies was developed as a surrogate for p53 variant analysis.26, 27 Several observations argued that p53 protein accumulation was not an intrinsic property of the TP53 protein.