Chromatin immunoprecipitation using anti-LEF-1 antibody using the lysates from NLK-low GBM cells pulled down the promoter DNAs of both CD44 and YKL40. In contrast, CHIP-PCR results using the lysates from NLK overexpressing cells were negative, suggesting that transcription of CD44 and YKL40 in these cells at least is regulated by NLK. This evidence concerns the gene LEF1 and glioblastoma.