The estimated frequencies of clonogenic cells were significantly high in the control tumor cells (1/4, 1/6, and 1/9 for 131, 387, and 827 cells, respectively) than NLK-overexpressing cells (1/95, 1/27, and 1/35 for 131, 387, and 827 cells, respectively), implicating the role of NLK in clonogenic growth of GBM (Figure 2C and 2D). Here, NLK is linked to neoplasm.