Finally, to determine whether NLK loss regulates mesenchymal activity through LEF-1, we determined the expression levels of CD44 and other mesenchymal markers in GBM cells expressing NLK alone, LEF-1 2A mutant, or both NLK and LEF-1 2A mutant (Figure 4M and 4N). Here, LEF1 is linked to glioblastoma.