We showed the advantage of our method in the quantification of methylation values of CRC markers NPY, PENK and WIF1, where, on undiluted stool DNA (100 ng) and by using the QM-MSP in presence of 1 mM spermidine, we globally enhanced detection by a near 10-fold factor, as assessed by summing up the three values. Here, PENK is linked to colorectal carcinoma.