As illustrated in Fig. 1A, in HEK293T cells transiently over-expressing CLC-1 WT or the myotonia-related A531V mutant, 24-hour treatment with 10 μM MLN4924 induced a significant increase in protein signal, suggesting that cullin-RING ligases may regulate CLC-1 protein expression. The gene discussed is CACUL1; the disease is Myotonia.