Remarkably, V1G1 was more enriched in GMB patients with higher sphere-forming ability (Figure 4B, 4C), and a lower ATP6V1G1 gene expression level (GBM#27 and 53 samples) was associated to reduced effect of V1G1 knockdown on sphere-forming ability of GBM cultures (Figure 4D). This evidence concerns the gene ATP6V1G1 and glioblastoma.