To test this hypothesis, we performed the aforementioned time-course infection studies with ExoT-expressing ∆U, ExoT/ADPRT-expressing ∆U/T(G-A+), and the T3SS mutant pscJ, and assessed the impact of ExoT or ExoT/ADPRT on FA sites by determining the total number of FAK and p-130Cas positive FA puncta per cell and the intensity of FAK and p-130Cas stainings per FA puncta by IF microscopy (described in Methods and S5 Fig). The gene discussed is PTK2; the disease is infection.