Screening revealed that, ovarian OVCAR-3, -4 and -5 and colon cancer HT-29, HCT-116 and SW620 over expressed both proteins OVCAR-5 CXCR4+CD133+ identified cells with cancer stem properties as demonstrated through in vitro spheres formation, clonogenic properties and stemness related genes expression (OCT-4, SOX2, KLF4, NANOG). Here, PROM1 is linked to colonic neoplasm.