Although this observation is at odds with our conclusion that infection with P. aeruginosa may reduce the efficacy of VX-809 + VX-770 in vivo, it is important to note that other bacteria in the CF lungs, including Acinetobacter nosocomialis and Acinetobacter baumannii secrete a virulence factor (aCif) that reduces CFTR abundance in CFBE cells [26], thus, it is possible that infection with other bacterial that are known to reduce CFTR abundance, in addition to P. aeruginosa, may influence the response to VX-809 + VX-770. Here, CFTR is linked to cystic fibrosis.