To further investigate the protein expression and correlation of survivin in human tissues, we conducted immunohistochemical analyses on survivin and other important kinases and prosurvival molecules, including p-Rb and p-S6, as well as autophagy related proteins, such as LAMP2 and LC3, through serial human HNSCC tissue arrays (n=43). This evidence concerns the gene RB1 and head and neck squamous cell carcinoma.