We validated the curve shift method by testing the combination of the PI3K inhibitor GDC-0941 and the MEK inhibitor AZD-6244 in colon carcinoma HCT 116 cells (Fig 1), a synergistic combination previously described by Haagensen et al. [38] following a biological discovery by Liu et al. [27]. The gene discussed is MAP2K7; the disease is colon carcinoma.