Our further in vivo study in spontaneously hypertensive rats (SHRs), a widely used animal model of essential hypertension, demonstrated pronounced antihypertensive effects of orally administered IRW that were likely mediated through several different pathways such as modulation of renin angiotensin system (RAS) through ACE inhibition, reduced vascular inflammation, increased nitric oxide (NO) mediated vasorelaxation though increased expression of endothelial nitric oxide synthase (eNOS) and NO bioavailability by reducing oxidative/nitrosative stress 18. This evidence concerns the gene NOS3 and hypertensive disorder.