A375 (human melanoma), SVR (murine angiosarcoma), and A2058 (human melanoma) cells treated with solenopsin A and analogs were evaluated by Western-blotting with p-Akt S473, p-MAPK 44/42, and B-actin (Additional file 1: Figure S4 and Additional file 1: Table S1). This evidence concerns the gene ACTB and melanoma.