The percent range of the Ki67-nuclei used for determination of tumor-to-normal ratio of Ki67 (i.e., rKi67) varied from 0.05−23.45 % in cancer tissues (n = 9), and from 0.19−7.41 % in normal tissues (n = 9) (see Additional file 1: Table S2). Here, MKI67 is linked to neoplasm.