The pro-tumourigenic activity of fibroblast-secreted HGF has also been reported in vitro. Conditioned media from isolated and activated HSC, pre-incubated with anti-HGF antibodies, was found to abrogate the proliferative and migration-inducing effects on HCC cell lines, seen in non-treated conditioned media [72]. This evidence concerns the gene HGF and hepatocellular carcinoma.