MAP1LC3B and posterior cortical atrophy: Inhibition of 4E-BP1 could sensitize PCa cells to rapamycin-induced autophagy [25, 26], so we studied autophagy in LNCaP and LNCaP-pc-1 cells using acridine orange staining, flow cytometry, and LC3B Western blot and immunofluorescence after rapamycin treatment (Fig. 6B, 6C, 6D, 6E and 6F) as described in Balakumaran's study [26].