PLAT and malaria: Here, we therefore studied the insertion of the EMCV IRES between an early promoter and the ATG start codon of a reporter transgene (rLuc), which is made by the fusion of tPA (tissue plasminogen activator leader sequence that directs the antigen to the secretory pathway of the cell) to pb9 (a strong malaria MHC class I H2-Kd-restricted epitope from the rodent malaria Plasmodium berghei circumsporozoite protein) and then to the Renilla luciferase gene.