S1D Fig confirms that in all five HCC cell lines tested, enhanced Elk-1 or MZF-1 increased transcriptional activity. The enhancement of both Elk-1 and MZF-1 were greater than that of Elk-1 or MZF-1 alone in terms of transcriptional activity. These results confirmed previous findings that PKCα expression is regulated by the two transcription genes[5–7]. The gene discussed is MZF1; the disease is hepatocellular carcinoma.