Moreover, the tight correlation was found between the metabolic activity ratio and protein reduction ratio (R2 = 0.996, P < 0.001) (Fig 6D, Table 3), and between the UGT1A4 metabolic activities of individual HLMs and the relative protein expression level in HBV-positive HCC tumor tissues (R2 = 0.828, P < 0.001) (Fig 6E, Table 4). Here, UGT1A4 is linked to hepatocellular carcinoma.