Notably, in two CRC patients we observed that Tim-3+PD-1−CD8+ T cells produced less IFN-γ than Tim-3−PD-1−CD8+ T cells, suggesting that Tim-3 expression alone defines a population of dysfunctional T cells, as previously reported for HIV-specific CD8+ T cells [17] and NY-ESO-1 specific CD8+ T cells [16]. This evidence concerns the gene IFNG and colorectal carcinoma.