Therefore, in this study, we wanted to investigate the antitumor activity of the orally bioavailable dual PI3K/mTOR inhibitor, NVP-BGT226 (BGT226), on a panel of hepatocellular carcinoma (Mahlavu, SNU475, SNU449, HepG2 and Hep3B) cell lines in either normoxia and hypoxia condition. This evidence concerns the gene MTOR and hepatocellular carcinoma.