We sought to address questions related to microglial morphology in neurodegenerative disease tissue: 1) Is microglia pathology seen only in the presence of amyloid or tau pathology, or can it be seen in other age-related neurodegenerative diseases?; 2) Is there microglial regional heterogeneity in the hippocampus (for example, gray matter only)?; and, 3) Can digital neuropathological quantification detect differences in microglia activation in different neurodegenerative diseases? The gene discussed is MAPT; the disease is neurodegenerative disease.