CEP250 and bone osteosarcoma: In order to provide a coherent picture on centrosome linkage and to understand the contribution of the centrosomal linker and microtubules on centrosome positioning in different cell lines, we analyzed RPE1, Human Bone Osteosarcoma Epithelial Cells (U2OS) and HeLa cells in combination with siRNA depletion of C-Nap1 and nocodazole treatment (S7 Fig).