In the present study, a detailed subtyping of AZFc structural mutations was performed using a complete experimental procedure including AZFc-STS (sequence tagged sites) detection, AZFc gene copy analysis, and dosage analysis of deleted in azoospermia (DAZ), chromodomain protein, Y chromosome, 1 (CDY1) and basic protein, Y chromosome, 2 (BPY2). The gene discussed is BPY2; the disease is Azoospermia.