When samples were further analyzed in the ID-Card 50531 (LISS/Coombs containing polyspecific AHG serum) to determine IgG- in addition to IgM-isoagglutinins, the isoagglutinin titers detected were higher as compared to the NaCl-system, but still no difference was observed between CVID patients and controls (Figure 1A, right panel). This evidence concerns the gene CD40LG and common variable immunodeficiency.