Our recent unpublished in vivo studies with the C57Bl/6 mice have now shown that systemic delivery of a mixture of two plasmids, prostate-specific Probasin-Cre/Tf-PEG-PEI-nanoparticles and floxed pSico-CD44v9shRNA/Tf-PEG-PEI-nanoparticles, can target both localized and metastatic prostate cancer cells. The gene discussed is TF; the disease is Familial prostate cancer.