To accomplish this feat, we performed a side-by-side activation of CLL B-cells cells and healthy donor B-cells with 10ug/ml anti-IgM for 1, 2, and 4 hours to determine the precise AKT threonine 308/4E-BP1 serine 65 phosphorylation pattern in CLL. This evidence concerns the gene CD40LG and B-cell chronic lymphocytic leukemia.