In this study we found that the expression of pAKT (Ser473) decreased after treatment with vemurafenib and anti-225D9+-TT Abs in hypoxic conditions (Fig. 6B) confirming that hypoxia affected the response to vemurafenib and anti-225D9+-TT Abs in 518A2 melanoma cells. This evidence concerns the gene DDX41 and melanoma.