By means of ROC curve analysis we showed a good sensitivity and specificity of CD4+ peripheral frequency to predict ischemic stroke (AUC = 0.964, P = 0.0001; cutoff value > 41.2%, sensitivity = 90.9, specificity = 95.5; see Figure 1); in regard to the peripheral frequency of CD28− cells ROC curve analysis demonstrated good sensitivity and specificity to predict stroke (AUC = 0.880, P = 0.0001; sensitivity = 70.4, specificity = 98.5, cutoff value > 4.1%, (see Figure 1). The gene discussed is CD28; the disease is stroke disorder.