Treatment with the mTOR inhibitor rapamycin did not affect AMPK phosphorylation; however, incubation with two distinct AMPK activators, AICAR and alpha lipoic acid, prevented both the insulin resistance (impaired insulin-stimulated Akt phosphorylation) and mTOR/p70S6K phosphorylation. The gene discussed is RPS6KB1; the disease is Insulin resistance.