To investigate the exclusive role of p53 in NP/RNF43 interaction governed apoptosis, p53 null human colon cancer cells, HCT116 (p53−/− HCT116) were transfected with pCDNA3.1 or pCDNA3.1-RNF43-Flag-HA with or without pEGFPN1 or pEGFP-NP. Here, RNF43 is linked to malignant colon neoplasm.