The statistical analysis showed that the relative level of PLA2G16 mRNA expression in metastatic primary osteosarcoma tissues (mean ± SD: 3.65 ± 0.60) was clearly higher than that in non-metastatic tissues (mean ± SD: 2.49± 0.69; P < 0.05, Fig 1). This evidence concerns the gene PLAAT3 and osteosarcoma.