Positive PLA2G16 IHC staining was mainly present in the cytoplasm of the tumor cells (Fig 2A–2H).Of the 119 osteosarcoma patients, positive PLA2G16 expression was observed in 65 patients (54.6%).The PLA2G16 expression levels in the whole tumor sections were entirely consistent with the results from the TMA analysis. Here, PLAAT3 is linked to neoplasm.