Because of the critical roles for CerS6 and CerS2 in liver homeostasis and insulin sensitivity [48], and cell membrane integrity [50], changes in C16:0 and C24:0 ceramide observed in our study may be involved in insulin resistance and development of NAFLD in LDLR-/- mice. The gene discussed is LDLR; the disease is metabolic dysfunction-associated steatotic liver disease.