The analysis of mTORC1-activity in patient tissue should be performed with caution since a high number of tumors also show strong expression of phospho-RPS6 (Ser235/236), phospho-RPS6 (Ser240/244) and phospho-4EBP1 (Thr37/46) on non-neoplastic cells in CNS tumors. This evidence concerns the gene RPS6 and central nervous system neoplasm.