RPS6 and intrahepatic cholangiocarcinoma: While the treatment of LNT-229 cells with torin2 almost led to eradication of the ICC signal, we observed a reduction of specific staining in rapamycin-treated cells only with the antibodies against phospho-RPS6 (Ser235/236) and phospho-RPS6 (Ser240/244) (Fig 2A and 2B).