The vaccine was able to expand survivin reactive cells even from myeloma patients with a low pre co-culture survivin specific precursor frequency (near or below the limit of detection of the LDA assay), and the survivin reactive precursor frequency of CD4+CD25- cells was not predictive of the fold expansion of survivin reactive with vaccine stimulation (r = −0.4857, p = 0.36 by Spearman correlation analysis). This evidence concerns the gene CD4 and plasma cell myeloma.