Clonogenic assays further showed that Notch-1 knockdown significantly suppressed the clonogenic growth of ACC-M cells (116 ± 7) compared with non-infected ACC-M cells (176 ± 13) or ACC-M cells infected with lentiviral vectors bearing scrambled siRNA (164 ± 12) (p < 0.01) (Fig. 4b, c). This evidence concerns the gene NOTCH1 and adrenal cortex carcinoma.