Our flow cytometry showed that Notch-1 knockdown was associated with a significantly higher proportion of late apoptotic cells (63.7 ± 3.8 %) compared to non-infected ACC-M cells (45.4 ± 2.6 %) or ACC-M cells infected with lentiviral vectors bearing scrambled siRNA (46.3 ± 2.7 %) (p < 0.01) (Fig. 6a and b). The gene discussed is NOTCH1; the disease is adrenal cortex carcinoma.