Our flow cytometric analysis revealed no statistically significant difference in the proliferation index of Notch-1 knocked down cells, non-infected ACC-M cells or ACC-M cells infected with lentiviral vectors bearing scrambled siRNA (p > 0.05) (Table 2, Fig. 5), indicating that Notch-1 downregulation did not impact on the cell cycle distribution of ACC-M cells. The gene discussed is NOTCH1; the disease is adrenal cortex carcinoma.