We found that Notch-1 knockdown by pLenR-Notch-1-shRNA1 significantly suppressed the proliferation of ACC-M cells compared with non-infected ACC-M cells or ACC-M cells infected with lentiviral vectors bearing scrambled siRNA cells (p < 0.01) (Fig. 4a). Here, NOTCH1 is linked to adrenal cortex carcinoma.