Immunohistochemical staining of tumor xenografts showed a marked reduction in the expression of Notch-1 in ACC-M cells whose Notch-1 was knocked down by pLenR-Notch-1-shRNA1 compared to non-infected ACC-M cells or ACC-M cells infected with lentiviral vectors bearing scrambled siRNA (immunoreactivity score: Notch-1 knockdown, 0.0 vs. scrambled siRNA, 5.0; p < 0.01) (Fig. 8c). Here, NOTCH1 is linked to neoplasm.