The two- to fourfold lower read-out/exon ratios observed for two of the four genes (FUS and HNRNPA2B1) in Δvhs infection resulted from higher total RNA levels compared with wild-type HSV-1 infection (Supplementary Fig. 3) reflecting the lack of vhs activity rather than reduced read-out36. Here, HNRNPA2B1 is linked to infection.