To further confirm this observation, Cy, a chemotherapeutic and immunosuppressive agent for the treatment of some neoplastic and autoimmune diseases (details in Materials and Methods), was administered to the wild-type and Robo1/2+/− knockout mice at 8 weeks of age because Cy has been reported to disturb follicle growth and toresult in premature menopause and sterility athigh doses16, 17, 18. Here, ROBO1 is linked to autoimmune disease.