The effect of MFN2 in cardiac hypertrophy was further elucidated using mice with cardiac-specific MFN2 knockout, where a loss of tethering to the ER may cause impaired Ca2+ signalling or enhanced ROS production (Chen and Dorn, 2013; Papanicolaou et al., 2011). This evidence concerns the gene MFN2 and cardiac hypertrophy.