In order to assess the functional consequences of CX3CR1 deficiency on microglial migration and the effect it has on tumor growth, we generated gliomas ex vivo in naïve organotypic slice cultures generated from B6, Cx3cr1GFP/+ and Cx3cr1GFP/GFP mice as described in Supplemental Figure 9. This evidence concerns the gene CX3CR1 and neoplasm.