Concurrently, overexpression of EFEMP1 in osteosarcoma cell lines promoted migration and invasion in vitro and vivo. The results showed that EFEMP1 behaved as a pro-tumor glycoprotein in osteosarcoma and strongly suggested that EFEMP1 might be involved in the progression and metastasis of osteosarcoma. The gene discussed is EFEMP1; the disease is osteosarcoma.