In addition, Western blot analysis revealed that 10 % GC-MSC-CM also led to increased phosphorylation of Akt or p44/42 MAPK (Erk1/2) in gastric cancer cells, which was partly abrogated by IL-8-specific neutralizing antibody treatment (Fig. 7E and Fig. 6G). The gene discussed is AKT1; the disease is gastric cancer.