Finally, NOTCH2 depletion in C8orf4-silenced Huh7 cells or HCC primary cells also abrogated the C8orf4 depletion-mediated enhancement of xenograft tumour growth (Fig. 4j), suggesting that C8orf4 acted as upstream of NOTCH2 signalling. The gene discussed is NOTCH2; the disease is hepatocellular carcinoma.