We found no significant publication bias for MTHFR C677T, A1298C and G1793A polymorphisms for overall risk of cancer, as suggested by funnel plots and Egger’s test results (P = 0.126 for C677T: CT/CC; P = 0.079 for C677T: CT+TT/CC; P = 0.474 for C677T:TT/CC; P = 0.950 for A1298C: AC/AA; P = 0.919 for A1298C: CC+AC/AA; P = 0.990 for A1298C: CC/AA; P = 0.383 for G1793T: GT/GG). This evidence concerns the gene MTHFR and cancer.