The break-apart and ASPL-TFE3 dual-fusion FISH assay can accurately detect the translocation of the TFE3 gene and ASPL-TFE3 fusion gene and can thus serve as a valid complementary method for diagnosing Xp11.2 tRCC and ASPL-TFE3 RCC. Here, ASPSCR1 is linked to renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.