Herein, we report a new break-apart FISH probe for diagnosing Xp11.2 tRCC and a novel ASPL-TFE3 dual-fusion FISH probe for diagnosing ASPL-TFE3 RCC. The gene discussed is ASPSCR1; the disease is renal cell carcinoma associated with Xp11.2 translocations/TFE3 gene fusions.