Intratumoral TLR3 positive rate was negatively related to the serum AFP levels (χ2 = 6.2411, P = 0.012) and positively related to HBsAg infection (χ2 = 9.5477, P = 0.002) and tumor with cirrhosis background (χ2 = 26.5278, P = 0.000). This evidence concerns the gene TLR3 and neoplasm.