To assess whether the previously documented phenotypic effects were due to SMYD3 methyltransferase activity, sh-SMYD3 PCa cells were transfected with mutant SMYD3 deleted for main components of SMYD3 SET domain, the EEL and NHSC amino acid sequences and compared with forced SMYD3 expression in sh-SMYD3 PC3 cells (Figure 5A). Here, SMYD3 is linked to posterior cortical atrophy.