When Treg cells were transferred at a time when inflammation was not yet present (i.e., at the same time as naive T cells), both control and Itgb8 KO Treg cells completely suppressed weight loss and the development of colitis (Figures S3A–S3D), in agreement with recent findings (Edwards et al., 2014) and the findings that integrin β8 expression on Treg cells does not play a function in preventing inflammation during homeostasis (Figure 2). This evidence concerns the gene ITGB8 and colitis.