MicroRNA-34a, which regulates the NAD+-dependent histone deacetylase SIRT1 as well, as HDAC1 and HDAC7 (2,34), was downregulated with simvastatin in all cancer cell lines investigated in this study, but most significantly in simvastatin-treated MDA-MD-231 cells (Table 3). This evidence concerns the gene SIRT1 and cancer.